Upload Local TEXT File: (fasta, gcg, and raw sequence formats)

Type or Paste a Query Sequence:(no comments, numbers okay)

Choose the Appropriate BLAST Program:
BLASTN - nucletide query to nucletide db BLASTP - protein query to protein db BLASTX - translated (6 frames) nucl. query to protein db TBLASTX - transl. (6 frames) nucl. query to transl (6) nt db TBLASTN - protein query to translated (6 frames) nt db BLAT - fast nucleotide query to genome db

Choose a Sequence Database:
Test
or

Options: For descriptions of BLAST options and parameters, refer to here
Output format: Gapped alingments Ungapped alingments
Comparison Matrix: BLOSUM62 BLOSUM100 PAM40 PAM120 PAM250
Cutoff Score(S value): Default 30 50 70 90 110
Word Length(W value): Default 15 14 13 12 11 10 9 8 7 6 5 4 3 2 Default = 11 for BLASTN, 3 for all others
Expect threshhold: Default 0.0001 0.01 1 10 100 1000
Number of best alignments to show: 1 10 25 50 100 200 400 800 1000
Filter options: On Off